Cucumber (Cucumis sativus L.) with heterologous poly--glutamic acid has skin moisturizing, whitening and anti-wrinkle effects
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES(2024)
摘要
Three genes involved in poly-gamma-glutamic acid(gamma-PGA)synthesis cloned from Bacillus licheniformis were transformed into cucumber for the first time. Compared with control, its water content increased by 6-14 % and water loss rate decreased by 11-12 %. In zebrafish and human skin experiments, the moisturizing effect of transgenic cucumber was significantly higher than that of CK, gamma-PGA and hyaluronic acid group. Transgenic cucumber reduced facial wrinkles and roughness by 19.58 % and 24.97 %, reduced skin melanin content by 5.27 %, increased skin topological angle and L -value by 5.89 % and 2.49 %, and increased the R2 and Q1 values of facial elasticity by 7.67 % and 5.64 %, respectively. The expressions of aqp3, Tyr, silv and OCA2 were down -regulated, eln1, eln2, col1a1a and col1a1b were up -regulated in zebrafish after treated with transgenic cucumber. This study provides an important reference for the endogenous synthesis of important skin care functional molecules in plants.
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关键词
Heterologous synthesis,gamma-PGA,Cucumber,Zebrafish,Human skin,Moisturization,Anti-wrinkle,Whitening
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