Coordination of iron ions with phycocyanin for an improved Fenton activity at weakly acidic pH

Development of biomolecules coordinated iron ions-based Fenton agents is highly desirable for chemodynamic therapy in term of demanded biocompatibility and enhanced Fenton activity at tumor microenvironmental pH of 6.5. Herein, phycocyanin (PC), the only FDA-approved natural coloring agent, was selected to coordinate with iron ions. The spectroscopic investigations disclosed that PC displayed pH-dependent spectral and conformational responses upon addition of Fe ions. As a result, the effective formation of Fe-PC coordination merely occurred at pH 7 due to a less folded polypeptide matrix of PC. The formed Fe-PC coordination exerted an enhanced Fenton activity at pH 6.5 as attested by 3, 3 ', 5, 5 '-tetramethlbenzidine assay and steady-state kinetic analysis. These findings not only provide fundamental insights of Fe-PC coordination but also highlight the potential biomedical significance of Fe-PC for severing as an effective Fenton agent in chemodynamic therapy.