Repeatability of Quantitative Brown Adipose Tissue Imaging Metrics on Positron Emission Tomography with 18F-Fluorodeoxyglucose in Humans

Brown adipose tissue (BAT) is a promising target for anti-obesity interventions. This prospective test-retest study assessed the repeatability of several important quantitative BAT metrics. After cold activation, 24 subjects underwent positron emission tomography (PET)/computed tomography (CT) and PET/magnetic resonance imaging (MRI), utilizing 18F-fluorodeoxyglucose. Repeat imaging occurred within 14 days per an identical protocol. BAT volumes were strongly correlated between sessions for PET/CT (intraclass correlation coefficient [ICC], 0.85) and PET/MRI (ICC, 0.82). BAT maximum lean-body-mass-adjusted standardized uptake values (SULmax) were also strongly correlated between sessions for both PET/CT (ICC, 0.74) and PET/MRI (ICC, 0.83). Much longitudinal variability in BAT metrics was likely due to biological factors intrinsic to BAT, whole-body metabolic fluctuations, or temporal differences in cold-activation efficacy, rather than imaging factors. Future studies utilizing these imaging metrics to track the response BAT to interventions should incorporate this variation into sample-size considerations and response criteria.