First-Order Derivative Spectrophotometric Method for Simultaneous Determination of Brinzolamide and Timolol Maleate in Ophthalmic Formulation

•Novel approach for simultaneous estimation of brinzolamide and timolol by first-order derivative UV spectroscopy.•This method is simple, eco-friendly, cost-effective, convenient, accurate and precise.•Successful applicability in validating content uniformity of the marketed ophthalmic preparations.•The most prominent and sophisticated green metrics provide assurance of method's sustainability.•Enabling the way to minimizing Industrial analytical hazard wastes.