Transition of organic matter from allochthonous to autochthonous alters benthic nutrient dynamics via microbial food webs

The impoundment of rivers by dams has significantly modified sedimentation patterns and trophic structures. As a result, the algal -derived organic matter (OM), as opposed to terrestrial -derived OM, plays an increasingly important role along the river -reservoir gradient. This study utilized water -sediment microcosms to explore the impacts of allochthonous and autochthonous OM deposition on benthic nutrient dynamics mediated by microbial food webs. Our results revealed that OM addition led to increased fluxes of NH4+ and CO2, with the highest flux induced by cyanobacteria OM, followed by diatom and allochthonous OM. N2 release flux was promoted by allochthonous and diatom OM deposition but inhibited by cyanobacteria OM deposition. The amendment of autochthonous OM increased the activity of dehydrogenase and urease, while allochthonous OM with a higher C/N ratio enhanced the catalytic abilities of polyphenol oxidase and beta-glucosidase. Furthermore, OM deposition significantly reduced microbial community richness and diversity, except for eukaryotic richness, and induced pronounced changes in bacterial and eukaryotic community structures. Allochthonous OM deposition stimulated the utilization of bacteria and protozoan on native OM, resulting in a positive priming effect of 26.78 %. In contrast, diatom and cyanobacteria OM additions exerted negative priming effects of -44.53 % and -29.76 %, respectively. Bayesian stable isotope mixing models showed that diatom OM was primarily absorbed by protozoan and metazoan, while cyanobacteria OM was more easily decomposed by bacteria and transferred to higher trophic levels through microbial food webs. In addition, bacterial ammonification accounted for 74.5 % of NH4+ release in the allochthonous OM deposition treatment, whereas eukaryotic excretion contributed separately 83.3 % and 83.1 % to NH4+ release in the diatom and cyanobacteria OM addition treatments. These findings highlight the significance of accounting for the regulatory capacity of OM deposition when studying benthic metabolism within river -reservoir systems.