Effects of amber LED on inflammatory and regulatory monocytes and lymphocytes

The primary objective of the present study was to assess the impact of amber LED photobiomodulation (PBM) on human monocytes and lymphocytes that were polarized into proinflammatory and regulatory/reparative phenotypes. Human leukocytes were polarized with LPS or LPS + IL -4 for 2 h and irradiated after 2 and 6 h with amber LED (590 nm). Cell absorbance spectrum and gene and protein expression of IL-1 beta, IL -6, IL -10, IL -17, TNF alpha and IFN gamma determined after 24 h. The results showed that irradiation did not significantly alter absorbance of non -polarized monocytes, whereas irradiated polarized monocytes presented reduction in absorbance in 625850 nm region. Irradiated monocytes polarized with LPS + IL -4 presented reduction in absorbance in 600-725 nm region compared to non -irradiated group. Irradiated non -polarized lymphocytes presented absorbance peaks between 650 and 820 nm not seen in non -irradiated group. No difference was found in absorbance pattern of polarized lymphocytes after irradiation. Irradiation led to reduction in protein synthesis of IL -6 and TNF alpha in monocytes polarized to proinflammatory phenotype and increase in production of IL -17 in lymphocytes. Irradiation reduced production of IL -10 in monocytes and lymphocytes polarized to immunoregulatory phenotype. In conclusion, amber LED modulates light absorbance and expression of important cytokines in inflammatory/ repair processes in monocytes and lymphocytes.