Reciprocal inhibition and competitive hierarchy cause negative biodiversity-ecosystem function relationships

The relationship between biodiversity and ecosystem function (BEF) captivates ecologists, but the factors responsible for the direction of this relationship remain unclear. While higher ecosystem functioning at higher biodiversity levels ('positive BEF') is not universal in nature, negative BEF relationships seem puzzlingly rare. Here, we develop a dynamical consumer-resource model inspired by microbial decomposer communities in pitcher plant leaves to investigate BEF. We manipulate microbial diversity via controlled colonization and measure their function as total ammonia production. We test how niche partitioning among bacteria and other ecological processes influence BEF in the leaves. We find that a negative BEF can emerge from reciprocal interspecific inhibition in ammonia production causing a negative complementarity effect, or from competitive hierarchies causing a negative selection effect. Absent these factors, a positive BEF was the typical outcome. Our findings provide a potential explanation for the rarity of negative BEF in empirical data. Using a dynamical consumer-resource model and controlled microbial colonization in pitcher plant leaves, the research explores the influence of niche partitioning and other ecological processes on biodiversity-ecosystem function relationships. A positive biodiversity and ecosystem function (BEF) is the typical outcome unless specific inhibitory or competitive interactions are present. The findings offer a potential explanation for the rarity of negative BEF relationships in nature.image