Quality assessment, HPTLC-DPPH, analytical quality by design based HPTLC method development for estimation of piperine in piper species and marketed formulations

•The eco-friendly method or green chemistry approach is employed by AGREE scale calculator.•Quality assessments of the plants is carried out by considering the ayurvedic pharmacopeia of India.•Analytical quality by design tools are utilized to develop quality imbibed HPTLC method.•ICH guidelines bases method validation was carried out for various parameters (Linearity, LOD, LOQ, Precision, specificity and Robustness).•The HP-TLC DPPH assay was carried out for anti-oxidant potential determinations.