Water-Induced Expanded Bilayer Vascular Graft with Good Hemocompatibility and Biocompatibility

Shape memory polymer (SMP) vascular grafts are promising interventional vascular grafts for cardiovascular disease (CAD) treatment; However, hemocompatibility and biocompatibility, which are the critical issues for the SMP vascular grafts, are not systematically concerned. Furthermore, the water-induced SMP grafts are more convenient and safer than the thermally induced ones in case of the bioapplication. Herein, in this work, the new water-induced expanded bilayer vascular graft with the inner layer of crosslinked poly(epsilon-caprolactone) (cPCL) and the outer layer of water-induced SMP of regenerated chitosan/polyvinyl alcohol (RCS/PVA) are prepared by hot pressing and programming approaches. The results show that the inner and outer layer surfaces of the prepared grafts are smooth, and they exhibit good interfacial interaction properties. The bilayer grafts show good mechanical properties and can be expanded in water with a diameter expansion of approximate to 30%. When compared with commercial expanded polytetrafluoroethylene (ePTFE), the bilayer graft shows better hemocompatibility (platelet adhesion, hemolysis rate, various clotting times, and plasma recalcification time (PRT)) and in vitro and in vivo biocompatibility, which thus is a promising material for the vascular graft. The hemocompatibility and biocompatibility, the critical issues for shape-memory polymer vascular stents, are not systematically concerned. Herein, a water-induced bilayer vascular stent is developed and its hemocompatibility and biocompatibility are systematically investigated. Compared with commercial expanded polytetrafluoroethylene, it showed the better hemocompatibility (platelet adhesion, hemolysis rate, various clotting times and plasma recalcification time) and in vitro and in vivo biocompatibility.image