Dataset describing the amino acid catabolism of Thermoanaerobacter pseudethanolicus

The dataset depicts the catabolism of branched-chain amino acids by Thermoanaerobacter pseudethanolicus in the presence of thiosulfate under different culture conditions. The results reveal that the strain can degrade all three branched-chain amino acids resulting in the production of their correspond-ing branched-chain fatty acids and branched-chain alcohols with the fatty acids always being the dominant product. The highest amounts of 2-methyl-1-butanol from isoleucine were at pH 6.5, liquid-gas ratio of 0.98, and at 20 mM thiosul-fate concentration. A kinetic experiment of the branched -chain amino acids was done in the presence of thiosulfate as are data on selected enzyme activities related to alcohols and aldehydes. Finally, an NMR study using (13)C1 methyl-1 -butyrate during the degradation of leucine in the presence of thiosulfate was done to prove that the (13)C1-methyl-1-butanol was indeed from its corresponding fatty acid. (c) 2023 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )