Novel silver nanoparticles modified by β-cyclodextrin and TiO2 as a SERS substrate for ultrasensitive detection of sisomicin

•The synthesized β-CD/TiO2@AgNPs was used as SERS substrate to detect sisomicin.•The EF of β-CD/TiO2@AgNPs for sisomicin was 1.48 × 106.•The SERS method had lower LOD and higher selectivity for sisomicin.•This method was successfully applied to detect sisomicin in simulated serum samples.