Mobilization of lipase and metal in microgel for biodiesel production and ring-opening polymerization

Lipase catalyzed reactions have wide applications in biological and industrial production. However, achieving rapid and stable catalysis from a single free lipase under different conditions remains a challenging goal. Here, we construct a polymer microgel immobilized lipase catalytic system (PPL), which is synthesized through radical polymerization. Then, metals could coordinate with multiple sites and graft from the surface of microgel. The lipase activity results showed that zinc and lipase microgel display the best catalysis efficiency and the zinc/ lipase microgel co-catalysis system showed rapid and stable catalysis at different temperatures and pH. The fast and stable catalysis under different conditions contributes to the interface structure of enzyme/metal microgel. Moreover, the zinc/lipase microgel could complete biodiesel production and ring-opening polymerization. Therefore, this interface system of lipase-metal microgel provides a new platform for rapid and stable ester catalysis under different conditions.