Development and application of the UVC LED-based spectrophotometer for high frequency online monitoring of nitrate concentration in municipal wastewater

Developing a fast, sensitive and stable sensor for online monitoring nitrate concentration is an urgent need for the optimization of denitrification processes in wastewater treatment plants (WWTPs). In this study, the miniaturized spectrophotometric nitrate sensor was developed using UVC light-emitting diodes (LEDs) coupled with AlGaN or SiC-based photodiodes. The use of novel SiC photodiodes could effectively eliminate UVB and UVA light in the 230 nm LED emission spectra. Replacing 235 nm UVC-LED with 230 nm UVC-LED could effectively increase the detection sensitivity but not the linear range that follows the Lambert-Beer's law. The influences of dissolved organic matter (DOM) on nitrate analysis could be corrected by a unified coefficient alpha across the NO3- -N concentration ranging in 0-50 mg/L. Across the investigated WWTP samples, the correction coefficient alpha was mainly determined by the fractions of humic substances with apparent molecular weight (MW) of 1.5-9.0 kDa, while the degradation of the large MW proteinaceous biopolymer resulted in dramatic variations of correction coefficient alpha along the WWTP A/A/O process. Microfiltration membranes with pore sizes <= 30 mu m could sufficiently eliminate the influences of particles in effluents on photometric nitrate analysis. The ultrafiltration and enhanced coagulation treatment could effectively remove the biopolymer fractions of DOM in wastewater, and thus alleviate its influences on sensitivity and accuracy of nitrate analysis. The spectrophotometric nitrate sensors were successfully applied for on-line monitoring NO3- -N concentrations in the influent and effluent of denitrifying biofilter in a municipal WWTP, providing high-frequency feedback signals to precise control of feeding external carbon source.