Interaction between membrane curvature sensitive factors SpoVM and SpoIVA in Bicelle condition
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS(2024)
摘要
SpoVM and SpoIVA are essential proteins for coat assembly in Bacillus subtilis. SpoVM is a membrane curvature sensor, specifically localized on the forespore membrane. SpoIVA is an ATP hydrolase that self-assembles by hydrolyzing ATP. In this work, SpoVM and its mutant SpoVMP9A were obtained by cyanogen bromide cleavage and reconstituted into bicelles. The purification of SpoIVA was achieved through a rigorous process involving NiNTA chromatography column and size exclusion chromatography. This study utilized Biacore to obtain a direct determination of the kinetic parameters of interaction between SpoVM (SpoVMP9A) and SpoIVA in Bicelle conditions.
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关键词
SpoVM,SpoIVA,Purification,Bicelles,Interaction,SpoVMP9A
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