Preparation of potential organic fertilizer rich in -polyglutamic acid via microbial fermentation using brewer's spent grain as basic substrate

Brewer's spent grain (BSG) is a main byproduct of the beer industry. BSG is rich in a variety of nutrients, and the search for its effective, high-value utilization is ongoing. Environmental probiotic factor gamma-PGA was produced by fermenting Bacillus subtilis with BSG substrate and the fermenting grain components were analyzed. The gamma-PGA yield reached 31.58 +/- 0.21 g/kg of BSG. Gas chromatography-mass spectrometry and non-targeted metabolomics analyses revealed 73 new volatile substances in the fermenting grains. Furthermore, 2,376 metabolites were upregulated after fermentation and several components were beneficial for plant growth and development (such as ectoine, acetyl eugenol, L-phenylalanine, niacin, isoprene, pantothenic acid, dopamine, glycine, proline, jasmonic acid, etc). These results show that it is possible to synthesize adequate amounts of gamma-PGA for use as a functional fertilizer.