Electrochemical Aptamer-based Sensor Construction on Functionalised Graphitic Carbon Nitride Screen Printed Carbon Electrode

In this study, graphitic carbon nitride (mGCN) was prepared from melamine and then functionalised with carboxyl groups using a simple grafting post-treatment method. It is then applied for the fabrication of anatoxin-a (ATX) specific aptamer on a screen printed carbon electrode (SPCE). The functionalised mGCN matrix (COOH-mGCN) provided a favourable environment for the immobilization of the ATX aptamer and conducting material for electron transfer from the electrolyte to the electrode. The structure of prepared COOH-mGCN was characterized by employing fourier-transform infrared spectroscopy (FTIR). Layer-by-layer fabrication of the aptasensor was electrochemically analysed. The performance of the aptasensor fabricated showed a peak current of 147 μA and resistance of 0.84 kΩ.