Separation of spring viraemia of carp virus from large-volume samples using immunomagnetic beads

Jing Hongli, Zhang Min, Gao Longying, Si Liegang,Wang Na, Ge Mingfeng, Xu Shengwei,Wang Jianping,Wu Shaoqiang

Archives of Virology（2023）

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摘要

A method for separation of spring viraemia of carp virus (SVCV) from large-volume samples using immunomagnetic beads (IMBs) coated with a polyclonal antibody against SVCV was developed. The optimum amount of IMBs was 2 mg in 100 mL. After IMB treatment, the detection limit of SVCV in reverse transcription quantitative PCR (RT-qPCR) was 103 times the 50

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