Preparation of hollow polymer fluorescent microspheres and its application in lateral flow immunoassay

The functionalized polymer microspheres were prepared by soap- free emulsion polymerization using potassium persulfate (KPS) as the initiator, glycidyl methacrylate (GMA) and styrene (St) as monomers. It was found that microspheres prepared by adding a trace amount of NaCl could be swelled by tetrahydrofuran (THF) to obtain monodisperse hollow microspheres with complete surfaces. The Eu(III) complex dye was then loaded into the microspheres shell by means of organic swelling, and immunochromatographic detection of alpha 1 microglobulin (alpha 1-MG) and beta 2 microglobulin (beta 2-MG) was performed.