Study on the efficacy and mechanism of Jintiange Capsules in the tre atment of osteoarthritis in rats

Abstract Background: To study the pharmacodynamic effect of Jintiange capsules (JTG) on collagenase-induced osteoarthritis (OA) in rats; Based on the chondrocytes model, to explore the mechanism of treating OA with JTG-containing serum. Methods: 1. 60 Sprague-dawley (SD) rats were used to establish OA model by type II collagenase. Knee joint diameter, lower limb load and pain threshold were measured. Then ELISA detection, micro-CT scanning and crocus solid green staining were performed. 2. Chondrocytes were extracted from SD rats. The effect of JTG on the migration of chondrocytes induced by il-1β was evaluated by Scratch Test. Chondrocytes were divided into control group, model group and JTG Group. RNA sequencing, nucleic acid sequence analysis, KEGG pathway enrichment analysis and qRT-PCR were performed after administration. Results: 1. Micro CT and safranine fast green staining showed that the number of bone trabeculae in JTG group increased significantly, arranged neatly. 2. The containing serum of JTG significantly increased the scratch healing rate of chondrocytes. GO and KEGG showed that the above differential genes were mainly enriched in immune system processes and TNF signaling pathways. RT-qPCRresults indicated that the TNF pathway and immune system regulation related genes were key pathways and genes in the treatment of OA. Conclusion: JTG effectively blocks the progression of articular cartilage through the TNF pathway and genes involved in immune system regulation.