The expression and Prognostic Value of IFIT3 in esophageal squamous cell carcinoma

Abstract Objective This study aims to clarify the expression of IFIT3 in human esophageal squamous cell carcinoma(ESCC) and further explore the relationship between IFIT3 expression and clinical pathological characteristics and prognosis of ESCC patients. Methods The target gene IFIT3 was screened through differential expression gene analysis, cluster analysis, enrichment analysis, and construction of a protein protein interaction network (PPI network), and then validated through clinical patient tissue RNA extraction and reverse transcription quantitative PCR. The Mann Whitney U test and Kaplan Meier analysis were used to investigate the correlation between the relative expression of IFIT3 and the clinical pathological information and prognosis of ESCC patients. Results GEO2R detected 279 differentially expressed genes in ESCC and paracancerous tissues. Cluster analysis and enrichment analysis showed that Cluster 4 played an important role in immune-related functions. PPI network showed that IFIT3 was the hub gene in the Cluster 4. Clinical patient tissue samples confirm the differential expression of IFIT3 in ESCC and paracancerous tissues. Mann-Whitney U test showed that the relative expression of IFIT3 was significantly correlated with clinicopathological information in patients with ESCC. Kaplan-Meier survival analysis showed that the disease-free survival time and overall survival time of patients with low expression of IFIT3 were significantly longer than those of patients with high expression of IFIT3, and the correlations were more significant in some subgroups. Cox proportional hazards model showed that lymph node metastasis was an independent risk factor for the prognosis of ESCC patients. Conclusion IFIT3 is differentially expressed in the cancerous and paracancerous tissues of ESCC, and the relative expression level of IFIT3 is highly correlated with the clinical pathological characteristics and prognosis of ESCC patients.