Spontaneous and site-specific immobilization of PNGase F via spy chemistry

Liang Zhang,Wenhui Wang, Yue Yang,Xiang Liu,Zhu Wang, Li Pi,Xin Liu,Song Wang

RSC Advances（2023）

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摘要

Recombinant PNGase F was spontaneously, site-specifically, and covalently immobilized onto magnetic nanoparticles based on Spy chemistry. The immobilized PNGasae F exhibited excellent deglycosylation activity, thermostability, and reusability.

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关键词

spy chemistry,site-specific

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