Clinical and Biochemical Assessment of Ozonated Olive Oil Mixed Bone Substitute in Treating Intra Bony Periodontal Defect- Randomized Clinical Trial

Abstract Objective The objective of this study is to investigate the effect of ozonated olive oil in treating intra bony defect by means of clinical and radiographic parameters, and gingival cervicular fluid BMP2 level. Materials and methods Thirty periodontitis patients with a deep intra-bony defect. Group I were treated with open flap depridment(OFD) and deproteinized bovine bone(DBB) alone, Group II were treated by OFD and placing DBB with ozonated olive oil, and Group III were treated by OFD and placing DBB with ozonated olive oil and further application of ozone 3 weeks after surgery topically. Clinical parameters were recorded at 3, and 6 months. Radiographic parameters were recorded before surgery and after 6 months. GCF of BMP2 level were recorded before surgery, 1, 3, 7, 14, 21days after surgery. Results according to clinical and radiographic parmeters, all groups showed no significant difference between groups. On the basis of GCF of BMP2 level, all groups showed a significant difference between groups in different intervals. Before study until week three, Group II and Group III showed a significant difference (p < 0.025*) according to the percentage change from Group I. Conclusion The use of ozonized olive oil in intra-bony defects increase GCF level and availability of bone morphogenic protein 2 significantly compared to the control non ozonized group. Clinical relevance ozonated olive oil is working on anabolic and catabolic mediator; it is antimicrobial, immunostimulating, ant hypoxic, and biosynthetic material