Computer-aided rational design strategy based on protein surface charge to improve the thermal stability of a novel esterase from Geobacillus jurassicus

Runfei Song, Jin Zhang, Mengyu Zhu,Lin Lin,Wei Wei,Dongzhi Wei

Biotechnology Letters（2024）

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摘要

Although Geobacillus are significant thermophilic bacteria source, there are no reports of thermostable esterase gene in Geobacillus jurassicus or rational design strategies to increase the thermal stability of esterases. Gene gju768 showed a highest similarity of 15.20

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关键词

Surface charge protein engineering,Thermostable esterase,Site-directed mutagenesis,Rational design

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