Preparation process optimization and characterization of Litinan-based drug delivery system

Abstract Adriamycin is an antitumor antibiotic, but its systemic side effects and large adverse effects in clinical application limit its wide application. In this study, we prepared Lentinan-oleic acid polymers by esterification reaction and optimized their preparation process by response surface methodology. ADM/LNT-OA micelles were prepared by self-assembly to alleviate the side effects associated with drug administration. The morphology and performance measurements of ADM/LNT-OA were investigated by particle size potentiometry, fluorescence spectroscopy, scanning electron microscopy, and transmission electron microscopy. The results showed that the reaction between Lentinan and oleic acid at pH 7 for 16 h at 25 °C was the best preparation condition for LNT-OA, and the degree of substitution of Lentinan could reach 53.6% with 97.5% yield. The ADM/LNT-OA micelles were spherical micelles of 164.2±11.4 nm with a potential of -30.9±1.5 mV, and their critical micelle concentration was 0.063 mg. In summary, our results suggest that the ADM/LNT-OA drug delivery system provides a basis for improving the efficacy and reducing the toxic side effects of the antitumor drug ADM.