ARPC3 affects the prognosis of patients with hepatocellular carcinoma by regulating the immune response

Abstract Background ARPC3 is associated with poor prognosis in patients with various cancers. However, the mechanisms by which it affects immunotherapy and prognosis in patients with hepatocellular carcinoma (HCC) remain unclear. Method The expression difference in ARPC3 between normal and HCC tissues and the effect of ARPC3 on prognosis were evaluated by using multiple databases. GSEA was used to predict the pathway by which ARPC3 affects HCC progression. Using TCGA database, the First Affiliated Hospital of Anhui Medical University (AHMU) database and ICGC database, the correlation between ARPC3, tumor infiltrating lymphocytes (TILs) and immune checkpoints was studied. To explore the effect of ARPC3 on immune checkpoint inhibitors (ICIs), We investigated the association of ARPC3 with immunotherapy-associated ferroptosis genes. Results The expression of ARPC3 in normal tissues was lower than that in tumor tissues, and as an independent prognostic risk factor for HCC, patients with HCC whose ARPC3 expression was high had a worse prognosis. GSEA suggested that the upregulation of ARPC3 mainly affected immune-related pathways. Three databases showed that ARPC3 expression levels affected the infiltration levels of B cells, T cells, macrophages, neutrophils, and NK cells in tumors. In addition, we confirmed that ARPC3 may influence the efficacy of ICI therapy by influencing the expression of immune checkpoints and ferroptosis-related genes in HCC. Conclusions ARPC3 is an independent prognostic risk factor for HCC patients and may influence the immunotherapy of HCC by influencing the expression of immune checkpoints and ferroptosis-related genes.