How Does Microtubular Network Assists in Determining the Location of Daughter Nucleus: Electromagnetic Resonance as Key to 3D Geometric Engineering

A dividing cell finds precisely the future 3D location to put its daughter cell by sensing the environment far outside its cell boundary. Making such a decision begins at a sub-molecular level of a pair of centrioles, eventually regulating the intricate geometries of a large life form. Thus far, optical imaging and molecular expression delivered little information. Here using theory and experiment, we propose that a scanning dielectric microscope (SDM) may predict the direction where parents would put their daughter with 65% (SD ± 5%) accuracy. The positioning mechanism of a microtubule organization center was monitored live using SDM of the 3D matrices of the hippocampal neuron and a HeLa cell network. We theoretically analyzed electric and magnetic field distributions at resonance for the relative 3D orientations of a pair of centrioles within a cell and also centrioles of the neighboring cells, beyond the optical range. Then microwave imaging revealed that all neighboring cell-centrosomes form a network of coupled vibrations that decides the left–right symmetry, symmetric, and asymmetric cell division. Together with Maxwell’s equation solver, SDM delivers deep insight into the multi-channel signal transmission in biomaterials beyond the optical microscope. For the first time, we combined two widely varied physical characterization tools to understand intelligence in biological systems.