Chirality Sensing with UV-visible Absorbance, Fluorescence, and Circular Dichroism Spectroscopy

Chirality-sensing techniques utilizing UV-visible, fluorescence, and circular dichroism spectroscopy are discussed in detail. The design strategies, applications, and limitations associated with these techniques are considered, as well as relevant historical details. For fluorescence and circular dichroism, brief introductions to the photophysics enabling sensing using these techniques are provided. Finally, we give a broad overview of the impact of these methods in the context of high-throughput experiments.