Çanakkale İli Kereviz (Apium graveolens L.) Yetiştirilen Alanlardaki Kök-ur Nematod (Meloidogyne spp. Goeldi, 1887) (Tylenchida: Meloidogynidae) Türlerinin Tanılanması

Identification of Root-knot Nematode (Meloidogyne spp. Goeldi, 1887) (Tylenchida: Meloidogynidae) Species in Celery (Apium graveolens L.) (Apiaceae) Growing Areas of Çanakkale Province ABSTRACT In this study, the species and extensiveness of root-knot nematodes (Meloidogyne spp. Goeldi, 1887) (Tylenchida: Meloidogynidae) causing yield loss in the celery (Apium graveolens L.) (Apiaceae) production areas of Çanakkale were determined. For this purpose, celery plant roots and soil samples were taken by making non-periodical surveys of a total of 75 different celery growing areas in Çanakkale province and districts in 2020-2021. Female individuals of root-knot nematodes and second-stage juveniles (J2s) from egg masses were obtained from celery samples brought to the laboratory. Morphological identification of root-knot nematode species was done by making sections obtained from perineal regions of female individuals and morphometric measurements were performed from J2s for each population. For molecular identification, DNA was then extracted from samples and analyzed by species-spesific primers referring to the most common Meloidogyne spp.. Meloidogyne javanica (Treub, 1885) Chitwood,1949 in 9 samples and M. arenaria (Neal, 1889) in 5 samples were determined based on morphologic -morphometric and molecular methods. As a result of the study, the rate of root-knot nematode infestation in celery cultivation areas in Çanakkale was determined as 18,6%. The infestation rates of M. javanica and M. arenaria which determined in celery growing areas in the province were 12% and 6.6%, respectively. In this study, M. javanica and M. arenaria species were detected for the first time in the celery fields of Çanakkale province. Keywords: Celery, Çanakkale, Prevalence, Root-knot nematode