Tackling PFAS Complexity with HRMS and Bioanalytical Techniques

Per- and polyfluoroalkyl substances (PFASs) are highly persistent anthropogenic compounds that are widespread in the environment. There are thousands of PFASs, yet few neat standards exist for unequivocal identification, quantification, or toxicity assays. The bioanalytical study of complex commercial PFAS mixtures is an innovative route to better understand novel PFAS exposure and toxicity. Here, we highlight efforts using high-resolution mass spectrometry (HRMS) and exposure-relevant mixtures to prioritize PFASs based on their potential to accumulate in living organisms.