High‐Throughput CRISPR/Cas9 Mediated Gene Editing of Primary Human T Cells in a Microfluidic Device for Cellular Therapy Manufacturing (Adv. Mater. Technol. 17/2023)

Michaela Welch, Deborah Flusberg,Peter Hsi,Nerses J. Haroutunian, J.P. Santos,Ernest S. Kim, Stacey Markovic, Jonathan Coppeta, Charles Lissandrello,Jenna L. Balestrini, Vishal R Tandon

Advanced Materials Technologies（2023）

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摘要

Cellullar Therapy Manufacturing T cells moving through a microfluidic device are electroporated in continuous-flow for high-throughput cellular therapy manufacturing, as reported by Vishal Tandon and co-workers in article 2300275. The combination of high throughput and high performance enables the scale of processing required for future “off-the-shelf” allogeneic cellular therapies.

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关键词

crispr/cas9 mediated gene editing,cellular therapy manufacturing,microfluidic device,human cells

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