Immunogenicity of a cold-chain friendly and long-term stable HIV multi-epitope protease cleavage sites (MEPCS)-mRNA-LNP vaccine.
Research Square (Research Square)(2023)
摘要
Abstract The failure of HIV vaccine clinical trials calls for novel vaccine development strategies. Studies on HIV highly exposed seronegative sex workers showed that focused virus-specific CD8+ T-cell responses are associated with the protection. Following the unconventional HIV vaccine approach targeting 12 highly conserved sequences surrounding the protease cleavage sites (PCS), we designed multi-epitope PCS mRNA-loaded lipid nanoparticles (MEPCS-mRNA-LNP) to promote more efficient antigen presentation. The modified LNP formulation produced stable and cold-chain-friendly MEPCS-mRNA-LNP. The mice study showed the vaccinated group generates PCS-specific CD8+ memory T-cells with cytotoxic function, both systemically and at the HIV-targeted tissue sites (like HESN group), with T-polyfunctional response. As expected, little to no significant activation of CD4+ T-cells was observed. The proof-of-concept study indicates that the MEPCS-mRNA LNP vaccine approach induces immunological memory against highly conserved HIV pro-polyprotein (limiting HIV’s mutability). The MEPCS-mRNA LNP vaccine could be a potential candidate for an effective prophylactic HIV vaccine.
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关键词
hiv,immunogenicity,vaccine,cold-chain,long-term,multi-epitope,mrna-lnp
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