Characterization of Lung and Oral Microbiomes in Lung Cancer Patients Using Culturomics and 16S rRNA Gene Sequencing

Yifan Sun,Yuejiao Liu,Jianjie Li,Yafang Tan,Tongtong An,Minglei Zhuo,Zhiyuan Pan,Menglei Ma,Bo Jia,Hongwei Zhang,Ziping Wang,Ruifu Yang,Yujing Bi

Microbiology spectrum（2023）

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摘要

The relationship between lung microbiota and cancer has been explored based on DNA sequencing; however, culture-dependent approaches are indispensable for further studies on the lung microbiota. In this study, we applied a comprehensive approach combining culturomics and 16S rRNA gene amplicon sequencing to detect members of the microbiotas in saliva and BALF samples from patients with unilateral lobar masses.

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oral microbiomes,rrna gene,lung cancer,lung cancer patients,cancer patients

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