Effect of hulled Cannabis sativa L. seeds in a home-made diet for adult dogs

Homemade diets integrated with functional ingredients may help to ensure that companion animals have a good life quality given the rise in their average lifespan. This study investigates the effects of a complete and well-balanced homemade diet supplemented with hulled hemp seeds. Twelve adult dogs divided into two groups: CTRL, fed the basal diet and HEMP, fed the diet integrated hulled Cannabis sativa L. seeds (4 g/100g of ration) were enrolled in the trial. The following samples were collected: (a) individual faecal samples to assess the diet digestibility through an indirect method of acid-insoluble ash; b) blood samples to evaluate the oxidative state through an OXI adsorbent test; and c) rectal swabs for intestinal microbiota analysis (alpha- and beta-diversity and taxonomy). The results reveal that with respect to the commercial diet used in this study, our homemade diet increased the total and protein digestibility (total: 53.97 ± 2.54% and 58.20 ± 2.58%; p < .030; protein: 70.54 ± 2.01% and 82.78 ± 5.35%; p < .001). The change in diet positively altered the microbiota structure and increased beta-diversity index significantly (p < .010). The hemp seeds significantly (p < .001) reduced the oxidative stress in the serum. This study highlights how hemp increases the body’s defences and that a homemade diet promotes diversity in the gut microbial population.