GREEN SYNTHESIS OF COPPER NANOPARTICLES MEDIATED FROM Coffee arabica SEEDS EXTRACT

The goal of this study is to synthesize copper nanoparticles (CuNPs) using Coffee arabica extract at room temperature using simple methods, characterize nanoparticle generation, and explore the impact of extract concentration on the production of copper nanoparticles, followed by a qualitative phytochemical screening of coffee extract metabolites and CuNPs toxicity demonstration. To characterize nanoparticle generation, color change, and UV-visible spectrophotometer were explored, analyzing by the Fourier Transform Infra-Red (FTIR) spectroscopy detected the availability of major functional groups, and the X-ray diffractometer demonstrated nanoparticles size. Additionally, CuNPs safety was demonstrated by hemolysis assays. The results revealed that the Coffee arabica beans solution shifted copper ions into copper nanoparticles at room temperature within 15 min of reaction time, and also registered peak absorbance at 262 nm in 3 days and 30 days to conform synthesis and stability CuNPs. In addition, FTIR analyses demonstrated the corresponding bonds (-OH, C=C, and C-H) that are responsible for the formation of CuNPs. Furthermore, the XRD results reported CuNPs to have a monoclinic crystalline shape with a mean crystallite size of 16.3 nm, CuNPs were found to be harmless in hemolytic experiments. Thus, this method can be used for optimum rapid synthesis of copper nanoparticles.