Proteomics analyses of five potential plasma-derived exosomal biomarkers for acute myocardial infarction

Abstract Acute myocardial infarction (AMI) is considered to be one of the most common cardiovascular complications. Recently, various research studies have shown that exosomes play a significant rolein the development and progression of cardiovascular diseases. However, there is still a lack of relevant research on the relationship between plasma exosomes and AMI. This retrospective study investigated the base date of patients with AMI(n = 20), stable angina pectoris (SAP, n = 20), and noncoronary heart disease (CON, n = 20). Proteomics was used to systematically screen the differential proteins of plasma exosomes in patients with clinical AMI, SAP, and CON. Then, the results were further verified by parallel reaction monitoring (PRM) and ELISA . Among the differential expression proteins, 5 proteins were quantified by PRM. Compared with the CON group, heparin cofactor 2 (SERPIND1), mannan-binding lectin serine protease 1 (MASP1), ficolin-2 (FCN2), and α1-Microglobulin/bikuninprecursor (AMBP)were upregulated in AMI and SAP, and they were more highly expressed in AMI than in SAP. Additionally, human leukocyte antigen (HLA-C) was found to be downregulated not only in exosomes, but also in plasma. The expression of four plasma exosomes biomarkersin AMI patients and stable angina pectoris (SAP) was higher than that in noncoronary heart disease (NCHD) patients, and HLA-C was found to be downregulated not only in exosomes, but also in plasma. The obtained results serve as anew candidate targetfor the detectionand therapyof AMI.