Flavonoid extraction from Siam weed (Chromolaena odorata) for the development of a wound-healing gel with antimicrobial properties

This study focused on extracting flavonoids, a group of natural compounds with high biological activity, from Siam weed (Chromolaena odorata). Various extraction conditions were investigated including the ratio of raw material to solvent, temperature, and extraction time. The highest flavonoid content was obtained when using ethanol with a concentration of 70% and a raw material-to-solvent ratio of 1:200 g/mL, at a temperature of 50oC for 2 h. Additionally, antibacterial activity test revealed that both the extract and gel (supplemented with the extract) were effective in limiting the growth of three bacteria strains including Staphylococcus aureus, Salmonella, and Escherichia coli. Therefore, this study suggests that this wild plant species may be a good source of natural antibacterial agents.