Method development for isolation and purification of Z-Guggulsterone, Dihydroguggulsterone, and Progesterone from guggul resin using RP-HPLC

Over thousands of years, oleogum resin (also known as guggul) from the Commiphora mukul tree, found in India, Bangladesh, and Pakistan, has been used to treat hypercholesterolemia, atherosclerosis, rheumatism, and obesity. The bioactive ingredient responsible for guggul's medicinal properties has been discovered as guggulsterone isolated from guggul. In the present work, Commiphora mukul components were obtained using bioactivity-guided fractionation. C. mukul exudates were extracted with ethyl alcohol, and the extract was subjected to column chromatography. Z-guggulsterone, dihydroguggulsterone, and progesterone were isolated by semi-preparative RP-HPLC. On the contrary, we isolated a mixture of E-guggulsterone and 3', 3''dimethoxysesamin from the fraction comprising the main components of different Sterones. 1H, 13C NMR, and LC-MS confirmed the compound's structure.