Microtubule-mediated cell shape regulation contributes to efficient cell packing inArabidopsis thalianacotyledons

Abstract Recent advances have started to uncover the mechanisms involved in organ and cell shape regulation. However, organizational principles of epidermal cells in different tissues remain poorly understood. Here, we show that polygonal representations of cotyledon pavement cells (PCs) in Arabidopsis thaliana exhibit increasing irregularity in side lengths and internal vertex angles during early stages of development. While the shape of PCs in cotyledons is more complex than that of cells in the shoot apical meristem (SAM), the polygonal representations of these cells share similar irregularity of side length. Comparison of the surface cell area with the area of the regular polygons, having optimally spaced tri-cellular junctions, reveals suboptimal junction placement for coverage in cotyledons and SAM. We also found that cotyledons show increased packing density compared to the SAM, indicating that PCs forgo coverage of larger areas to potentially increase tissue stability. The identified shape irregularity and cell packing is associated with microtubule cytoskeleton. Our study provides a framework to analyze reasons and consequences of irregularity of polygonal shapes for biological as well as artificial shapes in larger organizational context. Summary We provide a polygonal cell representation in a tissue context and use it to draw conclusions about cell packing in epidermis of A. thaliana cotyledon.