Mass Spectrometry-Based Approaches for the Analysis of Protein Higher Order Structure

Journal of Visualized Experiments(2023)

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摘要
Espino, J. A., Jones, L. M. In vivo hydroxyl radical protein footprinting for the study of protein interactions in Caenorhabditis elegans. Journal of Visualized Experiments. (158), e60910 (2020). Chea, E. E., Rinas, A., Espino, J. A., Jones, L. M. Characterizing cellular proteins with in-cell fast photochemical oxidation of proteins. Journal of Visualized Experiments. (157), e60911 (2020). Moorthy, B. S., Iyer, L. K., Topp, E. M. Mass spectrometric approaches to study protein structure and interactions in lyophilized powders. Journal of Visualized Experiments. (98), e52503 (2015). Habibi, Y., Thibodeaux, C. J. A hydrogen-deuterium exchange mass spectrometry (HDX-MS) platform for investigating peptide biosynthetic enzymes. Journal of Visualized Experiments. (159), e61053 (2020). Kirsch, Z. J., Arden, B. G., Vachet, R. W., Limpikirati, P. Covalent labeling with diethylpyrocarbonate for studying protein higher-order structure by mass spectrometry. Journal of Visualized Experiments. (172), e61983 (2021). Johnson, D., Punshon-Smith, B., Espino, J. A., Gershenson, A., Jones, L. M. Platform incubator with movable XY stage: A new platform for implementing in-cell fast photochemical oxidation of proteins. Journal of Visualized Experiments. (171), e62153 (2021). Haupt, C. et al. Combining chemical cross-linking and mass spectrometry of intact protein complexes to study the architecture of multi-subunit protein assemblies. Journal of Visualized Experiments. (129), e56747 (2017). Lento, C. et al. Time-resolved electrospray ionization hydrogen-deuterium exchange mass spectrometry for studying protein structure and dynamics. Journal of Visualized Experiments. (122), e55464 (2017). Weinberger, S. R., Chea, E. E., Sharp, J. S., Misra, S. K. Laser-free hydroxyl radical protein footprinting to perform higher order structural analysis of proteins. Journal of Visualized Experiments. (172), e61861 (2021). Misra, S. K., Sharp, J. S. Enabling real-time compensation in fast photochemical oxidations of proteins for the determination of protein topography changes. Journal of Visualized Experiments. (163), e61580 (2020). Chaihu, L. et al. Capillary electrophoresis-based hydrogen/deuterium exchange for conformational characterization of proteins with top-down mass spectrometry. Journal of Visualized Experiments. (172), e62672 (2021).
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关键词
protein higher order structure,spectrometry-based
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