Formulation and Evaluation of Probiotics Properties of Lactobacillus with Antimicrobial Activities

This study emphasizes exhibiting probiotics activities of Lactobacillus, formulation as granules, synthesizing nanoparticle by using Lactobacillus and demonstrating antimicrobial activity. Probiotics are not only microbes present in milk but also as are functional food to heal diseases. Four different isolates were obtained from sheep milk. These microbe’s growth profile was observed in different animal milk, and some biochemical studies were conducted. All four isolates were tested against gram positive and negative microorganisms like Salmonella typhi, Escherichia coli, Proteus vulgaris, Enterococcus faecalisis, Staphylococcus aureus etc. The nanoparticles of TiO2 was developed by using LAB via green synthesis. Granules of probiotics were prepared by using maltodextrin as agent by a lyophilization drying technique. The flow properties were studied for the prepared granule as formulation. The colony forming unit observed in case milk of buffalo was higher (12* X 109 CFU ± 2.5) which was observed in the other milking animals milk. The four isolates were identified as Lactobacillus (LAB) by Gram staining, motility catalase activity, along with other biochemical assays. The mimicking in-vitro simulation study showed higher survival rate in the intestine, demonstrated by bile salt studies. Microbes as a whole showed a good zone of inhibition as compared to the supernatant. Finally, these isolates have proven to be a good probiotic candidate that can be used as a natural antibiotic agent as a nutraceutical nominee. Granules exhibit good flow properties in case of all LAB formulations. The green synthesis of nanoparticles from LAB was observed at the absorption band at 405 to 410 nm using the UV method. LAB extracted from sheep milk was proven in the current investigation exhibiting as a potential candidate for the future probiotic antibacterial agent that resists GI environment.