Integrated Approach with Small-angle Scattering and Component-separation Techniques for Structure of Biomacromolecules in Polydisperse and Multi-component Solutions

Small-angle X-ray/neutron scatterings (SAXS/SANS; collectively called SAS) offer the structure of a biomacromolecule under near a physiological condition. To reveal a structure of a single biomacromolecule with SAS, it is indispensable to acquire the scattering profile which does not include the contribution of aggregates and other components. In this manuscript, we introduce two state-of-the art methods, Size Exclusion Chromatography-SAS (SEC-SAS) and Analytical UltraCentrifugation-SAS (AUC-SAS), for realizing the selective observation of the concerned component in polydisperse and/or multi-component biomacromolecular solutions.