Deletion of the AMPylase mFICD alters cytokine secretion and affects cognitive plasticity in vivo
bioRxiv (Cold Spring Harbor Laboratory)(2021)
摘要
Abstract Fic domain-containing AMP transferases (fic AMPylases) are conserved enzymes that catalyze the covalent transfer of AMP to proteins. This post-translational modification regulates the function of several proteins, including the ER-resident chaperone Grp78/BiP. Here we introduce a mFICD AMPylase knock-out mouse model to study fic AMPylase function in vertebrates. We find that mFICD deficiency is well-tolerated in unstressed mice. We show that mFICD-deficient mouse embryonic fibroblasts are depleted of AMPylated proteins. mFICD deletion alters protein synthesis and secretion in splenocytes, including that of IgM and IL-1β, without affecting the unfolded protein response. Finally, we demonstrate that older mFICD -/- mice show improved cognitive plasticity. Together, our results suggest a role for mFICD in adaptive immunity and neuronal plasticity in vivo .
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关键词
ampylase mficd alters,cognitive plasticity,cytokine
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