Development of Aged Skin Equivalent Model

Background: Skin aging is a result of the aging process and also intrinsic and extrinsic factors. In order to better understand this process and evaluate anti-aging products, it is necessary to develop in vitro models that can recapitulate the biological process of aged skin. Therefore, the aim of this study was to develop an aged skin equivalent model to evaluate these properties. Methods: Human fibroblasts were incorporated into the collagen matrix and keratinocytes were added and cultured in an air-liquid interface for 21 days. During this period, the matrices were exposed to UV or EX527 to trigger biological aging processes. Results: The two protocols evaluated demonstrated reduced expression of genes related to longevity and regulation of cellular redox homeostasis, and it was confirmed with histological analysis. Also, the model demonstrated the anti-aging potential of resveratrol. Conclusion: The model developed is a promising platform for reproducing aged skin and evaluating rejuvenating agents.