The Effect of Water Extract of Betaone against Osteoporosis in OVX mice by Inhibiting MAP Kinase Signaling

Abstract Betaone barley ( Hordeum vulgare ) is one of the most important crops and have detoxification, antioxidant, and cholesterol control effects. To analyze the effect of Betaone water extract (B1W) on osteoclasts, mRNA and protein expression of nuclear factor-activated T cells c1 (NFATc1) were measured. The mRNA expression of several proteins involved in osteoclast differentiation were investigated, and bone pit assays to investigate bone resorption function were performed. Finally, micro-CT imaging of ovariectomized (OVX) mice was performed to investigate in vivo activity. The results showed that B1W reduced the transcription and translation expression of NFATc1, and decreased mRNA expression levels of various marker genes and reduced the osteoclast activity. Moreover, improvement effects of B1W were obtained in osteoporosis-induced mice. Based on the results, B1W is considered a useful candidate as a therapeutic agent for treating bone diseases such as osteoporosis, and could also be used as an ingredient in health supplements.