Front Cover Picture: Engineering CYP153A_M.aq to Oxyfunctionalize its Inhibitor Dodecylamine Using a LC/MS Based Rapid Flow Analysis Screening (ChemCatChem 6/2022)

The Front Cover shows an alkylamine molecule about to enter an enzyme through its substrate tunnel, which is currently under construction. In their Research Article, L. R. Rapp et al. describe dodecylamine as a P450 wild-type inhibitor that could be accepted as a novel substrate by engineering the enzyme's entrance tunnel using a simple MS-based rapid-flow analysis technique. By combining experimental and computational work, the effects of the mutations on the substrate tunnel architectures were demonstrated. The results demonstrate the great potential of enzyme engineering beyond the active site to open the catalytic space to new groups of molecules or even alter inhibition. More information can be found in the Research Article by L. R. Rapp et al.