Cloning of the full-length coding sequence of tree shrew CD127 and prediction of its molecular characteristics

Objective Tree shrews is a good human diseases models.The cloning of its immune cellular markers is necessary.This study is to obtain Tree shrews CD127 for analyzing its molecular characteristics.Methods The CD127 gene was amplified from total RNA of tree shrews peripheral blood by RT-nested PCR and its biological characteristics were analyzed by biology software such as Discovery Studio.Results The fulllength CD127 cDNA encoding 1392bp of Tree shrews was cloned and one unknown fragment of tree shrews CD127 cDNA in the database was defined.Tree shrews CD127 amino acid sequence was conserved,and showed a close genetic relationship with Homo sapiens and Pan troglodytes.The structure of Tree shrews CD127 showed a similarity with human CD127,but some differences in the N-glyasylation sites number and the charge distribution have been detected.Conclusions The full-length sequence obtained in this study encodes Tree shrews CD127 which would function as its counterpart of the primate.It is useful for the preparation of antibody and studies of the role of Tree shrews Tr in human diseases. Key words: Tree shrews; CD127 ; RT-nested PCR; Structure; Monoclonal antibody