Expression of anti-HIV gene in Jurkat cell with Kaposi's sarcoma-associated herpes virus infection

Objective:To study the expression level of anti-HIV genes in KSHV (Kaposi’s sarcoma-associated herpesvirus) infected Jurkat cells.Methods: BCBL-1 cell was been stimulated by TPA (20ng/ml) 72 hours before havest KSHV virions solution.Jurkat cells were cultured with PRMI-1640 contain KSHV virions.24 hours after infection,The mRNA quantity of some anti-HIV genes in Jurkat cell infected with KSHV after 3 and 5 day’s were investigated by using Real-Time PCR.Results: After Jurkat cell infected with KSHV for 3 days,the mRNA production of RANTES,APOBEC3G,APOBEC3F,MX1,and MX2 increased 123,3.12,1.18,2.75,4.36 folds,respectively.In 5 days after infection,RANTES,MX1,and MX2 increased 11.91,2.72 and 2.22 times,respectively.Conclusions: the expression of anti-HIV genes(RANTES,APOBEC3G,APOBEC3F,MX1,MX2) up-regulated in KSHV infected Jurkat cells.