Pseudomonas aestuarii sp. nov., isolated from tidal flat sediment

Two novel Pseudomonas strains, SA3- 5T and SA3- 6, were isolated from a tidal flat (getbol) in the Republic of Korea. Strains SA3-5T and SA3- 6 were subjected to polyphasic characterization to determine their taxonomic affiliations. Cells were Gram- stain-negative, aerobic, rod-shaped and motile by using peritrichous flagella. Based on their 16S rRNA gene sequences, strains SA3-5T and SA3- 6 exhibited a high degree of similarity (100 %) and were classified within the genus Pseudomonas. Further-more, the closest related species to SA3- 5T and SA3- 6 were Pseudomonas taeanensis MS-3T (98.3 %). The ranges of average nucleotide identity and digital DNA-DNA hybridization values between SA3- 5T and closely related species were 75.9-89.1% and 21.3-38.7%, respectively, both of which being below the thresholds for delineating novel strains. Strain SA3- 5T and SA3- 6 contained C16:1 omega 6c and/or C16:1 omega 7c (summed feature 3), C16: 0 and C18:1 omega 6c and/or C18:1 omega 7c (summed feature 8) as the major fatty acids. The predominant respiratory quinone was Q-9. The DNA G+C content of strain SA3- 5T was 62.5 mol%. Based on their combined phenotypic, chemotaxonomic and phylogenetic characterisitics, strains SA3- 5T and SA3- 6 represent a novel species of the genus Pseudomonas for which the name Pseudomonas aestuarii sp. nov. is proposed. The type strain is SA3- 5T (=KCTC 92395T=JCM 35697T).