Lipopeptides as rhizosphere public goods for microbial cooperation

Cyclic lipopeptides (CLPs) are key bioactive secondary metabolites produced by some plant beneficial rhizobacteria such as Pseudomonas and Bacillus. They exhibit antimicrobial properties, promote induced systemic resistance in plants, and support key developmental traits, including motility, biofilm formation, and root colonization. However, our knowledge about the fate of lipopeptides once released in the environment and especially upon contact with neighboring rhizobacteria remains limited. Here, we investigated the enzymatic degradation of Bacillus and Pseudomonas cyclic lipopeptides by Streptomyces. We observed that Streptomyces venezuelae is able to degrade the three lipopeptides surfactin, iturin, and fengycin upon interaction with Bacillus velezensis in vitro and in planta according to specific mechanisms. S. venezuelae was also able to degrade the structurally diverse sessilin-, tolaasin-, orfamide-, xantholisin-, and putisolvin-type lipopeptides produced by Pseudomonas, indicating that this trait is likely engaged in the interaction with various competitors. Furthermore, the degradation of CLPs is associated with the release of free amino and fatty acids used by Streptomyces to sustain growth. Additionally, we hypothesize that lipopeptide-producing rhizobacteria and their biocontrol potential are impacted by the degradation of their lipopeptides as observed with the polarized motility of B. velezensis, avoiding the confrontation zone with Streptomyces and the loss of antifungal properties of degraded iturin.