Digital CRISPR/Cas13a-RPA based assay for rapid and sensitive detection of JC virus in renal transplant patients

Urine sample was obtained from renal transplant patient and then JCV DNA was extracted (A). Subsequently, RPA-Cas13a mix and oil were prepared for droplet generation at a microfluidic platform (B). After incubation at 37℃ for 30 min, the droplets were transferred to a detection chip for digital readout on a fluorescence detector (C). As shown, the digital CRISPR/Cas13a-RPA based assay can distribute the target DNA into nanoliter-sized droplets, thus enables the absolute quantification of the JCV in an isothermal environment.